Figure 6

Effect of beclomethasone on the bacterial growth of the Mm Δerp mutant. The Mm Δerp mutant strain was injected intravenously at 28 hpf, and at 44 hpi, the bacterial burden (A), the number of Mm clusters (B), the percentage of macrophages that contained 1-10 or more than 10 bacteria (of all macrophages containing bacteria) (C), and the percentage of Mm inside macrophages (D) were determined. No significant difference was observed between the vehicle- and beclomethasone-treated groups for any of these parameters. Statistical analysis was performed using two-tailed t-tests. Values shown are the means ± s.e.m. of three independent experiments, with each data point representing a single embryo. Statistical significance is indicated by: ns, non-significant. (E) Representative confocal microscopy image of Mm Δerp bacterial clusters (bacteria in green), indicated are clusters containing 1-10 bacteria and clusters containing more than 10 bacteria. Scale bar = 20 μm. (F) Representative images of the tail regions of a vehicle- and a beclomethasone-treated embryo infected with Mm Δerp bacteria. Scale bar = 100 μm.