Fig. 7

Single confocal plane of dazl^ae57/+ or dazl^ae57/ae57 8-14-day gonad labeled with Vasa and fluorescently conjugated phalloidin, which marks actin-rich structures and DAPI. (A,A′,C,C′, E,E′,G,G′) The actin-rich structures (white arrowheads) are present transiently in dazl^ae57/+ 8-12-day gonads. Number of gonads analyzed: 8 days, n=1 (A,A′); 10 days, n=1 (C,C′); 12 days, n=3 (E,E′); 14 days, n=2 (G,G′). (B,B′,D,D′,F,F′,H,H′) Actin-rich structures (white arrowheads) are present in 8-14-day dazl mutant GCs. (H,H′) At 14 days, actin-rich doublets are observed. Number of gonads analyzed: 8 days, n=1 (B,B′); 10 days, n=2 (D,D′); 12 days, n=2 (F,F′); 14 days, n=6 (H,H′). (I) Quantification of cells containing an actin-rich structure within each gonad. (J) Quantification of actin aggregates per cell. Error bars represent s.d.