PUBLICATION

Zebrafish dazl regulates cystogenesis and germline stem cell specification during the primordial germ cell to germline stem cell transition

Authors

Bertho, S., Clapp, M., Banisch, T.U., Bandemer, J., Raz, E., Marlow, F.L.

ID

ZDB-PUB-210317-5

Date

2021

Source

Development (Cambridge, England) 148(7): (Journal)

Registered Authors

Bandemer, Jan, Banisch, Torsten, Marlow, Florence, Raz, Erez

Keywords

Dazl, Fertility, Germline cyst, Germline stem cells, Incomplete cytokinesis, Ring canals

MeSH Terms

Cytokinesis/physiology
Animals
Fertility/genetics
Fertility/physiology
Gene Knockout Techniques
Germ Cells/growth & development*
Germ Cells/metabolism*
Zebrafish Proteins/genetics
Zebrafish Proteins/metabolism
Female
Mutagenesis
RNA-Binding Proteins/genetics*
RNA-Binding Proteins/metabolism*
Stem Cells/metabolism
Zebrafish/embryology
Zebrafish/genetics*
Zebrafish/metabolism*
Male

(all 18)

PubMed

33722898 Full text @ Development

Abstract

Fertility and gamete reserves are maintained by asymmetric divisions of the germline stem cells to produce new stem cells or daughters that differentiate as gametes. Before entering meiosis, differentiating germ cells (GCs) of sexual animals typically undergo cystogenesis. This evolutionary conserved process involves synchronous and incomplete mitotic divisions of a GC daughter (cystoblast) to generate sister cells connected by intercellular bridges that facilitate exchange of materials to support rapid expansion of the gamete progenitor population. Here, we investigated cystogenesis in zebrafish and found that early GCs are connected by ring canals, and show that Deleted in azoospermia (Dazl), a conserved vertebrate RNA-binding protein (Rbp), as a regulator of this process. Analysis of dazl mutants revealed Dazl's essential role in regulating incomplete cytokinesis, germline cyst formation, and germline stem cell specification before the meiotic transition. Accordingly, dazl mutant GCs form defective ring canals, and ultimately remain as individual cells that fail to differentiate as meiocytes. In addition to promoting cystoblast divisions and meiotic entry, dazl is required for germline stem cell establishment and fertility.

Errata / Notes

Corrected by: ZDB-PUB-220219-20

Genes / Markers

Figures

Show all Figures

Expression

Gene	Antibody	Fish	Conditions	Stage	Qualifier	Anatomy	Assay	Figure
dazl	Ab1-dazl	AB	standard conditions	Days 21-29		germ line cell cytoplasm	IHC	Fig. 3
dazl	Ab1-dazl	AB	standard conditions	Days 14-20		germ line cell	IHC	Fig. 3
dazl	Ab1-dazl	AB	standard conditions	Prim-15	Not Detected	primordial germ cell	IHC	Fig. 3
dazl		dazl^ae57/ae57	standard conditions	Days 7-13		germ line cell cytoplasm	ISH	Fig. 10
dazl		dazl^ae57/ae57	standard conditions	Days 14-20		germ line cell cytoplasm	ISH	Fig. 10
dazl	Ab1-dazl	dazl^ae57/ae57	standard conditions	Days 7-13		germ line cell	IHC	Fig. 3
dazl		dazl^ae57/+	standard conditions	Days 7-13		germ line cell cytoplasm	ISH	Fig. 10
dazl		dazl^ae57/+	standard conditions	Days 14-20		germ line cell cytoplasm	ISH	Fig. 10
dazl	Ab1-dazl	dazl^ae57/+	standard conditions	Days 7-13		germ line cell	IHC	Fig. 3
dazl		dazl^mu12/mu12	standard conditions	Days 14-20		germ line cell cytoplasm	ISH	Fig. 10

1 - 10 of 19

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Phenotype

Fish	Conditions	Stage	Phenotype	Figure
dazl^ae57/+; dazl^mu12/+	standard conditions	Days 7-13	germ line cell nuclear envelope morphology, abnormal	Fig. 5
dazl^ae57/+; dazl^mu12/+	standard conditions	Days 45-89	gonad lacks all parts of type germ line cell, abnormal	Fig. 9
dazl^ae57/+; dazl^mu12/+	standard conditions	Adult	female organism absence of anatomical entity, abnormal	Fig. 9
dazl^ae57/+; dazl^mu12/+	standard conditions	Adult	male organism male sterile, abnormal	Fig. 9
dazl^ae57/+; dazl^mu12/+	standard conditions	Adult	whole organism lacks all parts of type gamete, abnormal	Fig. 9
dazl^ae57/+; dazl^mu12/+	standard conditions	Adult	whole organism lacks all parts of type germ line cell, abnormal	Fig. 9
dazl^ae57/ae57	standard conditions	Days 7-13	germ line cell dazl expression decreased amount, abnormal	Fig. 3
dazl^ae57/ae57	standard conditions	Days 7-13	germ line cell nanos2 expression decreased amount, abnormal	Fig. 10
dazl^ae57/ae57	standard conditions	Days 7-13	germ line cell cytoplasm dazl expression decreased amount, abnormal	Fig. 10
dazl^ae57/ae57	standard conditions	Days 7-13	germ line cell increased size, abnormal	Fig. 4

1 - 10 of 19

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Mutations / Transgenics

Allele	Construct	Type	Affected Genomic Region
ae34		Indel	dazl
ae57		Indel	dazl
mu12		Indel	dazl
sa20350		Point Mutation	chek2
uc1Tg	Tg(piwil1:EGFP)	Transgenic Insertion
zdf1		Point Mutation	tp53

1 - 6 of 6

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Human Disease / Model

Sequence Targeting Reagents

Target	Reagent	Reagent Type
dazl	CRISPR1-dazl	CRISPR

Fish

Fish
AB
dazl^ae34/ae34
dazl^ae57/ae57
dazl^ae57/+
dazl^ae57/+; dazl^mu12/+
dazl^ae57/+; dazl^mu12/+; uc1Tg
dazl^mu12/mu12
dazl^mu12/mu12; uc1Tg
dazl^mu12/+
dazl^mu12/+; uc1Tg

Antibodies

Name	Type	Antigen Genes	Isotypes	Host Organism
Ab1-dazl	polyclonal	dazl		Chicken
Ab1-lmnb1	polyclonal		IgG	Rabbit
Ab5-ddx4	polyclonal		IgY	Chicken
Ab7-ddx4	polyclonal			Rabbit

Orthology

Engineered Foreign Genes

Marker	Marker Type	Name
EGFP	EFG	EGFP

Mapping

Bertho et al., 2021 ZDB-PUB-210317-5 PMID:33722898

Zebrafish dazl regulates cystogenesis and germline stem cell specification during the primordial germ cell to germline stem cell transition

Bertho et al., 2021

ZDB-PUB-210317-5
PMID:33722898