FIGURE 4

Overexpression of PIK3CA increases the viability and colony formation of DHTS‐treated A2780 ovarian cancer cells. A, Ectopic expression of PIK3CA in A2780 cells. PIK3CA expression level of A2780 cells that was transfected with an empty vector (Vector) or with a vector encoding PIK3CA (PIK3CA‐OX) was detected by real‐time PCR and Western blotting. B, Effect of PIK3CA overexpression on proliferation in DHTS‐treated A2780 cells after indicated plasmid transfection by MTT assay. Cells were treated with various concentrations of DHTS (0, 2, 4, 6 and 8 μM) for 24 h. C, Colony formation of A2780 cells stably expressing indicated plasmids. Cancer cells were treated with DHTS (2 μM) for 48 h and in vitro cultured for another 2 weeks for colony forming. The surviving colonies were stained with crystal violet and counted with ImageJ (colony counter). Data represent means ± SEM of triplicate samples. *P < .05 and **P < .01 vs. untreated control or wild‐type