FIGURE

Figure 2

ID

ZDB-FIG-210411-37

Publication

Zallocchi et al., 2021 - Characterization of quinoxaline derivatives for protection against iatrogenic-induced hearing loss

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Figure 2

Qx28 treatment in neuromast hair cells. Representative images of 5 dpf Tg(brn3c:GFP) zebrafish pretreated with Qx28 (1 nM–300 μM) followed by coincubation with the different ototoxins. (A–F) Short-term effect (STE) of gentamicin 100 μM for 1 hour followed by 1-hour recovery in E3 water. (G–L) Long-term effect (LTE) of gentamicin 100 μM for 1 hour followed by 5-hour recovery in E3 water. (M–R) Neomycin 200 μM for 30 minutes followed by 1-hour recovery in E3 water. (S–X) CDDP 400 μM for 6 hours followed by 1-hour recovery. (Y) Vehicle control neuromast. GFP shown in green, otoferlin shown in red. Neuromasts were imaged employing a Zeiss LSM 800 confocal microscope with a 63× oil objective and Airyscan function. Scale bars: 7 μm (A–X), 5 μm (Y). (Z) Chemical structure of Qx, Qx28, and Qx66. For sample numbers see legend of Figure 3.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	s273tTg
Conditions:	chemical treatment by environment: cisplatin chemical treatment by environment: cisplatin chemical treatment by environment: gentamycin chemical treatment by environment: gentamycin chemical treatment by environment: neomycin chemical treatment by environment: neomycin (all 6)
Observed In:	neuromast hair cell
Stage:	Day 5

Phenotype Detail

Acknowledgments

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