FIGURE

Figure 3

ID
ZDB-FIG-210409-32
Publication
Han et al., 2021 - Zebrafish mafbb Mutants Display Osteoclast Over-Activation and Bone Deformity Resembling Osteolysis in MCTO Patients
Other Figures
All Figure Page
Back to All Figure Page
Figure 3

Abnormal cartilage and bone formation in mafbb mutants. (A) Alcian Blue staining of embryos at 5 dpf. A1-A3, ventral views; A1′-A3′, lateral views. Ceratobranchial pairs (cb); ceratohyal (ch); Meckel’s cartilage (mk); basihyal (bh), CHA (ceratohyal angel). (B) Summary of CHA in embryos at 5 dpf (n = 15 embryos per group). (C) Ventral views of Alizarin Red (AR) staining of larvae at 8 dpf. C1′ and C2′ are the enlarged images of the teeth region (n = 30 embryos per group). (D) MicroCT scans of adult zebrafish at 10 mpf. The hypurals in the red boxed area are used for analysis in E. (E) Summary of BV/TV for the bones in the boxed area in D (n = 3 zebrafish per group); mean grey-level intensities of the boxed area in D. (F) AR staining of adult zebrafish at 9 mpf. F1′–F3′″ are higher magnifications of boxed area in F1–F3. Mouths (F′), vertebral columns (F″) and caudal fins (F′″). The black arrows in F1′–F3′ point to the lower jaw; the black arrowheads in F2″-F3″ point to the neural arches; the blue arrowheads in F1′″–F3′″ point to hypurals 3–5 (H3–5); The blue arrows point to H1,2; N = 3 zebrafish per group. WT, wild type; HET, mafbbd11/+; HOM, mafbbd11/d11; BV, bone volume; TV, total tissue volume. Results in B and E are expressed as mean ± SEM, (* p < 0.05, **** p < 0.0001, t test).

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Observed In:
Stage Range: Day 5 to Adult

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Biomolecules