FIGURE

Fig. 4

ID
ZDB-FIG-210408-10
Publication
Zhang et al., 2021 - Contributions of biliary epithelial cells to hepatocyte homeostasis and regeneration in zebrafish
Other Figures
All Figure Page
Back to All Figure Page
Fig. 4

BECs act as the major contributor to hepatocyte regeneration after extreme injury to fibrotic liver (A) Experimental scheme illustrating the stage of EtOH and Mtz treatment in transgenic line Tg(lfabp:Dendra2-NTR). (B) Confocal projection images (3D imaging) showing the staining of extracellular matrix protein collagen 1 in the liver region (dashed lines) after DMSO, EtOH, Mtz, and Mtz plus EtOH treatment at R0h (arrows). (C) Confocal projection images (3D imaging) showing the co-immunostaining for Dendra2 and 2F11 in regenerating livers after EtOH and Mtz treatment from R0h to R72h. (D) Single optical images showing the co-immunostaining for Dnedra2 and DsRed in regenerating livers at R24h. Most of the Dendra2+ cells are 2F11 positive in Mtz and EtOH treatment (arrows). In EtOH treatment, the 2F11+ and Dendra2+ cells are not co-stained (arrowhead). Nuclei were stained with DAPI (blue). (E) Experimental scheme illustrating the stage of 4OHT and EtOH treatment to double transgenic line, Tg(tp1:CreERT2; β-actin:loxP-DsRed-loxP-GFP), from 4 dpf to 5 dpf and analysis at 6 dpf. Immunostaining for 2F11 and GFP on livers (2D imaging) showing tp1-CreER labels the 2F11-positive cholangiocytes specifically after 4OHT and EtOH treatment. Nuclei were stained with DAPI (blue). Quantification of the percentages of GFP+ among 2F11+ hepatocytes (EtOH, n=5; EtOH+4OHT, n=8). (F) Experimental scheme illustrating the stage of 4OHT, EtOH, and Mtz treatment to triple transgenic line Tg(tp1:CreERT2; lfabp:loxP-STOP-loxP-DsRed; lfabp:Dendra2-NTR) and analysis at R72h. Single optical images showing the co-immunostaining for DsRed and Dendra2 at R72h after 4OHT, EtOH, and Mtz treatment. Quantification of the percentage of the DsRed+ among the Dendra2+ cells in regenerating livers at R72h (EtOH+4OHT, n=10; EtOH+4OHT+Mtz, n=10). Numbers indicate the proportion of larvae exhibiting the expression shown. Scale bars: 100 μm. Data are represented as mean ±SEM. See also Figures S4 and S5.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ iScience