FIGURE 3

Comparison of robustness and organization of repetitive ChR2‐induced and continuous NMDA‐induced fictive swimming. ChR2‐induced and NMDA‐induced fictive swimming were recorded from spinalized Tg(vglut2a:Gal4ff)^nns20;Tg(UAS:ChR2(H134R)‐mCherry)^umn201 preparations. ChR2‐induced swimming was stimulated for 10 s with an inter‐stimulus period of 3 min. Corresponding 10 s‐long windows of NMDA‐induced fictive swimming were analyzed every 3 min. (a) Representative peripheral nerve voltage traces from the first (t = 0) and final (t = 57 min) timepoints of ChR2‐induced (top; blue line represents continuous 10 s blue light stimulus) and NMDA‐induced (bottom) fictive swimming. (b) Total number of bursts detected during each 10 s recording of ChR2‐induced (ChR2; black; plot also shown in Figure 2c) and NMDA‐induced (NMDA; red) fictive swimming. (c) Episodic Organization (EO) scores of ChR2‐induced (black) and NMDA‐induced (red) fictive swimming. Recordings of 10 s were grouped into bins defined by elapsed experimental time. Each bin contained 5 voltage recordings (total of 50 s/bin). Shaded regions and error bars represent SD. Asterisks indicate significant differences when comparing the first and final stimuli in b and bins in c; ns indicates that differences were not significant.