FIGURE 5

Apoptosis and oxidative stress were more severely enhanced in serpini1^−/− zebrafish group under CoCl₂ induced hypoxic injury. (A) AO staining of the apoptotic cells in the live image of WT and serpini1^−/− zebrafish under the treatment of 10 mM CoCl₂ at 4dpf. Scale bar:500 um. (B) MDA content in WT and serpini1^−/− under 10 mM CoCl₂ induced injury. The data are presented as the mean ± SEM, n = 100 per group. Three trails for the experiment. Statistical analyses were performed with Student’s t-test, ***p < 0.001 compared with WT, ns: no significant. (C) The level of GSH-Px in WT and serpini1^−/− under 10 mM CoCl₂ induced injury. The data are presented as the mean ± SEM, n = 100 per group. Three trails for the experiment. Statistical analyses were performed with Student’s t-test, **p < 0.01 compared with WT, ns: no significant. (D) The level of CAT in WT and serpini1^−/− under 10 mM CoCl₂ induced injury. The data are presented as the mean ± SEM, n = 100 per group. Three trails for the experiment. Statistical analyses were performed with Student’s t-test, ***p < 0.001 compared with WT, ns: no significant. (E) The level of SOD in WT and serpini1^−/− under 10 mM CoCl₂ induced injury. The data are presented as the mean ± SEM, n = 100 per group. Three trails for the experiment. Statistical analyses were performed with Student’s t-test, ***p < 0.001 compared with WT, ns: no significant.