FIGURE

FIGURE 4

ID

ZDB-FIG-210310-27

Publication

Ranawakage et al., 2021 - Efficient CRISPR-Cas9-Mediated Knock-In of Composite Tags in Zebrafish Using Long ssDNA as a Donor

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FIGURE 4

Comparison of donor DNA template types. (A) Donor DNA templates used in this comparison. (B) Knock-in (KI) allele-specific PCR amplification for the 5′ junction. lssDNA (a), PCR fragments (b), or plasmid DNA (c) was microinjected with 1.5 fmol of the RNP complex into the cytoplasm of one-cell stage zebrafish embryos, and genomic DNA was extracted from 20 individual zebrafish embryos. As a negative control, knock-in allele-specific PCR amplification was performed using uninjected zebrafish embryos (d). β-actin2 gene-specific PCR was performed to confirm DNA integrity.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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