Immunohistochemistry of myocilin in ocular structures of adult zebrafish. Fluorescent immunohistochemistry of iridocorneal dorsal angle (A,E), cornea (B,F) and retina (C,D,G) sections (14 μm) of wild-type and KO myoc adult zebrafish (7 months). Samples were incubated with an anti-myocilin primary antibody (TNT), followed by Cy2-conjugate goat anti-chicken IgY secondary antibody (green signals). The TNT antibody recognises the N-terminal part of myocilin protein. Expression is seen in the non-pigmented epithelium of the ciliary body and in the iris stroma and iris vessels (A), in the corneal endothelium (B), and in the ganglion cell layer in the retina (C,D) (arrowhead) in wild-type. Red signals correspond to tissue autofluorescence and blue signals correspond to DAPI nuclear staining. al: annular ligament. npe: non-pigmented ciliary epithelium; ibv: iris blood vessels; is: iris stroma. cep: corneal epithelium; cs: corneal stroma; ce: corneal endothelium glc: ganglion cell layer; ipl: inner plexiform layer; inl; inner nuclear layer; opl: outer plexiform layer; onl: outer nuclear layer; phl: photoreceptor layer. re: retina+/+: wild-type; −/−: myoc KO. The negative controls are shown in Figure S4.
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