Knockdown (KD) of TAR DNA-binding protein 43 (tdp-43) caused motor impairments and defective neuromuscular junction (NMJ) structure. (A) Representative images of one ventral root projection double labeled for sv2 (presynaptic marker, red) and αBTX (postsynaptic, green). mis-CTRL embryos showed extensive red and green colocalization when converted in spots by Imaris software, as can be seen from the yellow staining seen in the merged images. Scale bar equals 10 μm. (B) Quantification of orphaned αBTX clusters for somite and of orphaned sv2 pre-synaptic marker. These clusters described as “spots” were measured following 3D reconstruction of the ventral CaP axon extension by Imaris [52]. Each point in these graphs represents the number of orphan spots per axon extension. Six fish were used for this study. (C) Relative transcript levels of ach receptors α and γ, and muscle histone deacetylase 4 (hdac4) expressed as a percentage and normalized to tubulin. Error bars represent standard error of the mean (SEM). * p < 0.05. ns = not significant.
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