FIGURE

Figure 5

ID

ZDB-FIG-210301-146

Publication

Chrystal et al., 2021 - The Axenfeld-Rieger Syndrome Gene FOXC1 Contributes to Left-Right Patterning

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Figure 5

Loss of foxc1 does not significantly change cilia length or left–right organizer flow. (A) Acetylated α-tubulin immunostaining of left–right organizer (LRO) cilia revealed that average cilia length was not significantly altered in foxc1 mutants (foxc1a^−/−, 82%; foxc1b^−/−, 93%; foxc1a^−/−; foxc1b^−/−, 82% of WT length; p = 0.286, ANOVA; 4–11 embryos imaged per condition, all cilia per condition shown in light grey). Tracking of fluorescent bead flow in the LRO revealed unchanged counterclockwise flow between WT (B), foxc1a^−/− homozygotes (B’), and foxc1a/foxc1b morphants (B’’).

Expression Data

Antibody:	Ab1-tuba
Fish:	AB foxc1a^{ua1017/ua1017} (AB) foxc1b^{ua1018/ua1018} (AB) foxc1a^{ua1017/ua1017}; foxc1b^{ua1018/ua1018} (AB)
Anatomical Term:	Kupffer's vesicle cilium
Stage:	10-13 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	foxc1a^{ua1017/ua1017} (AB) foxc1b^{ua1018/ua1018} (AB) AB + MO1-foxc1b + MO2-foxc1a foxc1a^{ua1017/ua1017}; foxc1b^{ua1018/ua1018} (AB)
Knockdown Reagents:	MO1-foxc1b MO2-foxc1a
Observed In:	Kupffer's vesicle cilium Kupffer's vesicle epithelial cilium movement involved in extracellular fluid movement
Stage:	10-13 somites

Phenotype Detail

Acknowledgments

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