Fig. 4

Fig. 4. Recombinant zfIFNϕ1 decreases mortality, morbidity and viral load in TiLV-infected zebrafish larvae. Survival of zebrafish larvae (A). Larvae were injected with 1 ng of recombinant zfIFNϕ1 or PBS (final vol. of 1 nl) into the duct of Cuvier at 2.5 dpf. 6 h post-stimulation larvae were mock-infected or TiLV-infected by injection into the duct of Cuvier. Comparisons between survival curves were made using the log-rank test. Percentage of dead, ill or healthy larvae pre-treated with zfINFϕ1or PBS 6h before mock or TiLV infection (B). The procedure was the same as for Fig. 4A. The data in Fig. 4A and B are representative of two independent experiments. Normalized copy numbers of TiLV RNA in 5 pooled larvae of zebrafish injected with PBS (white bars) or recombinant zfIFNϕ1 (black bars) into the duct of Cuvier at 2.5 dpf (C). Larvae were infected with TiLV by injection into the duct of Cuvier 6 h post-stimulation. Data are normalized against mRNA transcript of ef1a gene of the host. The symbol (*) indicates significant differences between the non-stimulated control larvae and zfIFN1 stimulated larvae at each time point (*p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001) as revealed by two-way ANOVA followed by a Bonferroni test. Each bar represents the mean + SD of n = 9–10 samples derived from two independent experiments.