glyt1−/− mutants’ sensory ganglia are more active while arousal and motor circuits are less active than their siblings during recovery from propofol. (A) Brain regions of interest (ROIs) that are significantly different between glyt1−/− mutants and their siblings are shown on reference brains for Baseline , Propofol, 10 min. wash and 20 min. wash conditions with sample size indicated at the base of each image. ROIs are labeled with the corresponding anatomical brain regions; an abbreviation key can be found at the end of the figure legend. (B–D) Violin plots of pERK/tERK ratios for twelve different brain regions are shown with a dashed line indicating median pERK/tERK levels in baseline glyt1 siblings. For each brain region, we conducted a 2-way ANOVA for genotype and condition followed by Sidak’s multiple comparisons of genotype across conditions. Asterisks indicate p-value: *p < 0.05, **p < 0.001, ***p < 0.001, ****p < 0.0001. (A) Three sensory ganglia are shown that are elevated in glyt1 mutants during recovery. (B) Olfactory bulb and arousal pathways are shown. (C) Premotor and motor regions elevated in baseline glyt1 siblings are shown. (D) Hypnotic regions elevated in baseline glyt1−/− mutants are shown. Anatomical abbreviations: OE olfactory epithelium, subpallium SP, pallium P, Preoptic nucleus PO, Hypothalamic nucleus enriched in Qrfp-expressing neuronal cell bodies Qrfp, Ventral Thalamus VT, Dopaminergic Cluster 2 in the posterior tuberculum Dop 2, Tectum Stratum Periventriculare TSPv, Tectum Neuropil TN, Cerebellum CB, vagal motor nucleus X, posterior Lateral Line Neuromasts along the body LLN D. Scale bar = 100 µm.
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