FIGURE

Fig. 6

ID

ZDB-FIG-210205-10

Publication

Barone et al., 2020 - Designed nanomolar small-molecule inhibitors of Ena/VASP EVH1 interaction impair invasion and extravasation of breast cancer cells

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Fig. 6

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Fig. 6

Cellular and in vivo studies with the initial and optimized inhibitors. (A) IncuCyte chemotaxis assay with inhibitor-treated MDA–MB–231. (Left) Invasion is inhibited in a dose-dependent manner (representative phase object count, normalized to fitted IC50 curves with 3σ dashed line). (Right) IC50s were calculated during linear phase object signal increase of the assay and are plotted as a group. (B) MDA–MB–231 cancer cells extravasation in zebrafish treated with PBS (control) or inhibitors 1, 4a, 6, or 7 at 1 μM concentration. (C) Representative fluorescence images of zebrafish embryos taken on day 5. MDA–MB–231 (red) extravasate into the avascular tailfin (white arrows).

Expression Data

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Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA