FIGURE

Fig. 2

ID

ZDB-FIG-210204-24

Publication

Olson et al., 2020 - Lamellipodia-like protrusions and focal adhesions contribute to collective cell migration in zebrafish

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Fig. 2

Fig. 2. Arp2/3 activity is required for brush-like protrusion formation and pLLP migration. (A) Effects of increasing concentration of CK-666 on pLLP migration in comparison to vehicle and inactive control drug, CK-689. (B) Mosaically labeled embryos carrying TgBAC(cxcr4b:LifeAct-Citrine) transgene were first exposed to DMSO (vehicle) and imaged for 1.5 h beginning at 30 hpf (Movie 2). (C) Following DMSO treatment, embryos were then exposed to the Arp2/3 inhibitor CK-666 for 4 h and concurrently imaged (Movie 2). Note the loss of brush-like protrusions following the treatment. Images show lateral views. (B′,C′) 1.8X zoom of boxed cells in B and C. (D) Percentage of time a given cell contained a brush-like protrusion during vehicle and the inhibitor treatment (n = 11 cells from 6 embryos). (E) Migration speed of the whole pLLP during vehicle and inhibitor treatment (n = 8 whole pLLPs). Asterisks = brush-like protrusions. Scale bar = 10 μm.

Expression Data

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Antibody Labeling

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Acknowledgments

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Reprinted from Developmental Biology, 469, Olson, H.M., Nechiporuk, A.V., Lamellipodia-like protrusions and focal adhesions contribute to collective cell migration in zebrafish, 125-134, Copyright (2020) with permission from Elsevier. Full text @ Dev. Biol.