FIGURE

Fig. 1

ID

ZDB-FIG-210202-9

Publication

Galang et al., 2020 - ATAC-seq Reveals an Isl1 Enhancer that Regulates Sinoatrial Node Development and Function

Other Figures

All Figure Page

Back to All Figure Page

Fig. 1

Figure 1. Isolation of neonatal pacemaker cells (PC) for assay for transposase-accessible chromatin (ATAC-seq).A, Isolation of neonatal PCs and right atrial cardiomyocytes (RACMs) from the sinoatrial node (SAN) using fluorescence-activated cell sorting (FACS) on samples derived from 3 biological replicates, where each biological replicate contained pooled SAN tissue from 5 neonatal mice. B, Ventral view of an Hcn4 (hyperpolarization-activated cyclic-nucleotide-gated ion channel 4)-green fluorescent protein (GFP)/Myh6-mCherry neonatal heart with SAN positive for both fluorescent markers (yellow). C, Enrichment of known PC genes (green bars) and depletion of known RACM genes (red bars) from bulk RNA sequencing on sorted PCs and RACMs (each biological replicate is shown [PC1, PC2, and PC3], data are displayed as log(2) fold change of SAN/RACM transcripts per million [TPM]). Blue bars show genes that are not differentially expressed. D and E, ATAC-seq signal for individual PC (n=4) and RACM (n=3) biological replicates at Hcn4 locus (D) and Nppa locus (E) Each biological replicate consisted of pooled SAN tissue from 1 to 2 litters of neonatal hearts. la indicates left atrium; lv, left ventricle; ra, right atrium; rv, right ventricle; and svc, superior vena cava.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Circ. Res.