Fig. 2

Figure 2. Proximity of differential assay for transposase-accessible chromatin (ATAC-seq) peaks to differentially expressed genes and enrichment of cardiac TF (transcription factor) motifs and binding sites at ATAC-seq peaks.A, Clustered heatmap with Z scores for the top 500 differentially accessible (DA) ATAC-seq peaks across all 7 samples. B, Gene ontology terms for genes assigned to DA ATAC-seq peaks. C, Motif enrichment in DA ATAC-seq peaks. Motifs and false discovery rates (FDR) are for the underlined factors, encompassing several TF types, including LIM homeodomain proteins Isl1 (Islet-1), Lhx3 (LIM homeobox 3), and Lhx1 LIM homeobox 1); myocyte-specific enhancer factor 2 proteins; T-box (T-box transcription factors), Gata (GATA-binding proteins); and the TALE (3-amino acid-loop extension)-class proteins Meis1 (myeloid ecotropic viral insertion site 1), and Tgif2 and Tgif1 (TGFβ-induced factors 2 and 1). D, Overlap of published Mef2C (myocyte-specific enhancer factor 2C), Tbx5 (T-box transcription factor 5), Gata 4 (GATA-binding protein 4), Nkx2.5 (NK2 homeobox 5), and Shox2 (short stature homeobox 2) chromatin immunoprecipitation and sequencing peaks with DA ATAC-seq peaks. Abnl indicates abnormal; AP, action potential; Decr, decreased; RACM, right atrial cardiomyocytes; and SAN, sinoatrial node.