FIGURE

Fig. 3

ID

ZDB-FIG-210125-20

Publication

Loughland et al., 2021 - DNA methyltransferase 3a mediates developmental thermal plasticity

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Fig. 3

DNMT3a modulates thermal performance curves. A significant interaction between genotype and developmental temperature manifests as a progressive decline in swimming performance (U_crit) (a) of warm-developed fish (red symbols and lines) from control to DNMT3aa^−/− and to DNMT3aa^−/−ab^−/− where U_crit was lowest. The genotype x developmental temperature interaction determining citrate synthase (CS) activity (b) results from higher activity in warm-developed DNMT3aa^−/− fish compared to cold-developed fish. As for U_crit, activity of warm-developed DNMT3aa^−/−ab^−/− fish was lowest but similar to control. Marginal means (± s.e.) are shown across both acclimation treatments in a and b. Sample sizes were n = 12 for U_crit (24 for marginal means shown) and n = 8–9 for CS activity (16–18 for marginal means). Developmental temperature interacted with acclimation temperature to determine U_crit (c), and CS activity (d); marginal means (± s.e.) across all genotypes and test temperatures are shown

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	dnmt3aa^{zf3452/zf3452} (AB/TU) dnmt3aa^{zf3452/zf3452} + CRISPR1-dnmt3ab (AB/TU)
Condition:	cold exposure
Knockdown Reagent:	CRISPR1-dnmt3ab
Observed In:	whole organism citrate synthase activity whole organism swimming behavior
Stage:	Adult

Phenotype Detail

Acknowledgments

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