Fig. 2

Parental acclimation rescues offspring survival. Fecundity of F₁ fish (a) reared at 28 °C and acclimated to 23 °C did not differ significantly between genotypes (control = grey circles, DNMT3aa^−/− green circles, and DNMT3aa^−/−ab^−/− purple circles). Genotype or developmental temperature had no effect on F₂ early survival rates (b). There was a small but significant increase in deformity rates in DNMT3aa^−/− larvae at 23 °C (indicated by asterisk) (c). There was no significant effect of genotype or developmental temperature on late survival rates (d). Each circle in a–d represents an individual clutch (n = 17–32 clutches per treatment), and means ± s.e. are shown (black horizontal and vertical lines, respectively). Multiple dimension scaling plot (e) of reduced representation bisulphite sequencing results showed no global differences in methylation pattern between control and DNMT3aa^−/−ab^−/− fish from both developmental temperatures (triangles = 28 °C developmental temperatures, inverted triangles = 23 °C, grey triangles = control, purple triangles = DNMT3aa^−/−ab^−/−)