FIGURE

Fig. 3.

ID

ZDB-FIG-210113-129

Publication

de Vos et al., 2020 - The novel zebrafish model pretzel demonstrates a central role for SH3PXD2B in defective collagen remodelling and fibrosis in Frank-Ter Haar syndrome

Other Figures

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Fig. 3.

Adult sh3pxd2b^Δ/Δ zebrafish develop cutaneous fibrosis that worsens over time. Representative H&E and PSR stained transverse sections at the level of the dorsal fin of sh3pxd2b^Δ/Δ mutant fish and sh3pxd2b^+/+ WT clutch mates at the ages of 3, 5 and 11 months, selected from five assessed fish per genotype per time point, imaged with a Zeiss AxioImager Z.2 slide scanner. At 3 months of age, transverse sections of mutants (C,D) and their WT clutch mates (A,B) are indistinguishable. At the age of 5 months, mutants have accumulated an increased amount of connective tissue at the base of the dorsal fin between the lepidotrichiae (G, solid arrow heads) and around the distal radial (outlined arrow head) as compared to their WT clutch mates (E), primarily consisting of collagen (F,H). At 11 months of age, the base of the dorsal fin in mutants is wider than their WT clutch mates and completely filled with collagen (I–L). In addition, collagen accumulates in the stratum compactum (arrows), myosepta (asterisks), and between individual muscle fibres (hash signs) in mutants. Scale bars equal 500 µm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	sh3pxd2b^{zf3422/zf3422} (AB)
Observed In:	dermal deep region dermal deep region Collagen dorsal fin distal radial Collagen lepidotrichium myoseptum myoseptum Collagen
Stage:	Adult

Phenotype Detail

Acknowledgments

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