Fig. 6

Light-Sheet Laser Masks. A. User Interface used to define laser masks allowing the user to display and use a number of masks for recordings: Edge masks help eliminate excess light during laser return as visible in D (with Edge masks enabled) compared to C (without any masks), Eye Mask allowing to turn the laser off in a specific region (such as eye of larval zebrafish) and Blank Return which turns the laser off during return (flyback), avoiding unnecessary photobleaching of the sample. B The median-normalized luminance intensity profiles of a light sheet in Fluorescein with no mask applied (blue, shown in C), edge masks (red, shown in D) and eye mask (green, shown in E). F. An illustration of how the ‘edge’ and ‘eye’ masks could be used in the context of imaging the brain of a larval zebrafish while minimizing illumination of the retina.