FIGURE

Figure 7

ID
ZDB-FIG-201229-70
Publication
Keefe et al., 2020 - Vanishing white matter disease expression of truncated EIF2B5 activates induced stress response
Other Figures
All Figure Page
Back to All Figure Page
Figure 7

Human EIF2B2 gene rescues zebrafish eif2b2sa17223/sa17223 mutants. (A) 11.1 kb zebrafish eif2b2 gene structure. (B) 9.5 kb human EIF2B2 gene structure. (C) Conservation of amino acid sequence between zebrafish eif2b2 and human EIF2B2. (D) Schematic of rescue construct containing Tol2, β-actin, human EIF2B2, and eGFP. (E) Genotype results (at adult age), of an eif2b2sa17223/+ heterozygous incross, and an eif2b2sa17223/+ heterozygous zebrafish crossed with two different transgenic alleles (#3 and #4): eif2b2sa17223/+;Tg3(β-actin:EIF2B2:2A:eGFP) or eif2b2sa17223/+;Tg4(β-actin:EIF2B2:2A:eGFP) heterozygous zebrafish. (F) Bright-field and immunofluorescent images of eif2b2sa17223/sa17223;β-actin:EIF2B2:2A:eGFP mutant fish: showing a swim bladder and regular-sized head; or showing GFP expression in eif2b2sa17223/sa17223;β-actin:EIF2B2:2A:eGFP mutant fish. (G–J) TUNEL and DAPI antibody staining of wild-type control; eif2b2sa17223/sa17223 mutant; eif2b2+/+;β-actin:EIF2B2:2A:eGFP wild-type control; and eif2b2sa17223/sa17223;β-actin:EIF2B2:2A:eGFP mutant. (K) Quantification of TUNEL+ cells.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Observed In:
Stage: Day 6

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Elife