FIGURE

Fig. 4

ID

ZDB-FIG-201202-10

Publication

Loontiens et al., 2020 - PHF6 Expression Levels Impact Human Hematopoietic Stem Cell Differentiation

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Fig. 4

PHF6 modulates Notch1 expression and its downstream signaling activity. (A) (left) Flow cytometry analysis of control and PHF6 shRNA transduced CD34⁺ thymocytes in OP9-DLL1 cocultures in the presence of IL7, SCF and FLT3L, showing the development of CD4⁺CD8β⁺ DP thymocytes after 21 days of coculture. (right) Bar plot showing the frequency of CD4⁺CD8β⁺ DP thymocytes, generated in the corresponding cultures. Data shows the average of 4 independent experiments and errors bars show SEM. *P < 0.05 (paired t-test) (B) Normalized NOTCH1 and DTX1 expression in Jurkat cells (left) and CB-derived CD34⁺ HPCs (right) following control or PHF6 shRNA transduction as indicated. Data shows the average expression in 3 independent samples and error bars indicate SEM.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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