Fig. 3

Tools available in zebrafish to facilitate spatiotemporal study of miRNA targets at a cellular level. a–c Ventral view confocal images of the craniofacial cartilages of Tg(4.9Sox10:EGFP;col2a1aBAC:mcherry) (a), Tg(col2a1aBAC:mcherry;col10a1aBAC:citrine)hu7050 (b), Tg(- 4.9Sox10:EGFP; 4xGTIIC:eGFP) (c) imaged live. Scale bar = 100 μm. d’, d’’ Ventral view confocal images of the craniofacial cartilages of Tg(Sox10:GAL4- VP16; UAS:Kaede) with photoconverted chondrocytes shown in magenta and annotated with a white arrowhead in the same fish at 4 dpf (d’) and 5dpf (d’’). Scale bar = 100 μm. e’–e’’ Confocal images of the lower jaw joint in Tg(Sox10:GAL4- VP16; UAS:Kaede) with photoconverted chondrocytes shown in magenta and annotated with a white arrowhead in the same fish at 3 dpf (e’) and 5dpf (e’’). Scale bar = 50 μm, images in e’ and e’’ previously published in (Brunt et al. 2017). f, g Ventral view confocal images of the craniofacial cartilages of Tg(5xBMPRE-Xla.Id3:GFP; col2a1aBAC:mCherry) (f) and Tg(7xTCF.XlaSiam:nlsGFP;col2a1aBAC:mCherry) (g) imaged live. Scale bar = 100 μm. h Finite element model of maximum principal (E. max) strains in 5dpf wild-type zebrafish viewed from a dorsal orientation. E. max represents tensional strains with cooler colours (blue) on the scale corresponding to lower strain and red corresponding to higher strain values. MC Meckel’s cartilage, JJ jaw joint, PQ palatoquadrate, C ceratohyal