Fig. 2.1

(A) Pretectum area of a 7 dpf Tg(ath5:mRFP;NeuroD:GCaMP6f) fish. NeuroD-driven expression of GCaMP6f (green) includes pretectal neurons with cell bodies in close proximity to AF7, that expresses mRFP (red) under the ath5 promoter. (B) Composite GCaMP6f fluorescence time courses for prey-experienced (N = 13) and prey-naïve (N = 7) fish associated with eye convergence (vertical black line) for brain areas and behavioral measures shown in Figure 2H. Fish selected to have > 5 eye convergences in presence of prey (evoked). Activity in the contralateral tectal neuropil is higher than in the ipsilateral tectal neuropil in evoked but not spontaneous condition. Difference between amplitude at peak convergence between contralateral and ipsilateral is significant for prey-experienced (p = 0.049) and prey-naïve (p = 0.02) fish. ‘Contra’ and ‘ipsi’ refer to the side with higher or lower pretectal transient amplitude peak time (see Materials and methods). (C, D) Quantification of evoked (in presence of paramecia) forebrain activity in (B) for telencephalon (1) and habenula (2) over five frames (1.4 s) after eye convergence. We observe higher activity in prey-experienced fish than in prey-naïve fish in both the telencephalon (C) and the habenula (D) (p = 0.025 and p = 0.009), respectively (permutation test, see Materials and methods, Behavioral data analysis and statistics). Data tables for panels B, C and D in Figure 2—figure supplement 1—source data 1.