FIGURE

Fig. 4

ID

ZDB-FIG-200921-4

Publication

Zhu et al., 2020 - The Joubert Syndrome Gene arl13b is Critical for Early Cerebellar Development in Zebrafish

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Fig. 4

Disruption of arl13b reduces both precursor and differentiated cerebellar Purkinje cells. A–I Representative images of in situ hybridization using anti-sense probes against ptf1a and roraa to label Purkinje precursors and differentiated cells, respectively. The dorsomedial clusters of precursor of Purkinje cells and differentiated Purkinje neurons are selectively reduced (arrows). J–L’’ Immunostaining of mature Purkinje neurons using anti-parvalbumin antibody reveals that the dorsomedial Purkinje neurons are reduced in arl13b-deficient embryos. A–I, Scale bar 100 μm. J–L’’ Scale bar 50 μm.

Expression Data

Genes:	ptf1a roraa
Antibody:	Ab1-pvalb
Fish:	AB arl13b^{hi459Tg/hi459Tg} AB + MO1-arl13b
Knockdown Reagent:	MO1-arl13b
Anatomical Terms:	Purkinje cell Purkinje cell layer corpus cerebelli Purkinje cell layer corpus cerebelli dorso-medial region Purkinje cell layer corpus cerebelli Purkinje cell
Stage Range:	Long-pec to Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	arl13b^{hi459Tg/hi459Tg} AB + MO1-arl13b
Knockdown Reagent:	MO1-arl13b
Observed In:	cerebellar Purkinje cell differentiation Purkinje cell Purkinje cell layer corpus cerebelli Purkinje cell layer corpus cerebelli dorso-medial region
Stage Range:	Long-pec to Day 5

Phenotype Detail

Acknowledgments

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