Figure 5

(A) Casper zebrafish larvae were treated at 60 hr post-fertilization (hpf) with either vehicle control or the indicated protective agents. At 72 hpf, larvae were rinsed then treated with either vehicle control or 0.125 mM cisplatin for 24 hr. Larvae were then injected via the common cardinal vein with FITC-inulin, then measured for fluorescence swiftly with the Biosorter. Larvae were rinsed then measured 2 hr later. Fold change in overall larval fluorescence is represented in relation to 0 hr. ****=p<0.001, as per two-way ANOVA with a Tukey post-test. Three replicates, with 50 larvae/treatment group/time point minimum. Representative images of larvae can be found in Figure 5—figure supplement 1d. Larvae were treated as in (A) and were fixed at either 24 hr post-treatment (B–E) or at 72hpt (Figure 5—figure supplement 2a-d). Larvae were pre-embedded in low melting point agarose, then in paraffin, then sectioned and stained with H and E. (B) Control, (C) Cisplatin only, (D) Cisplatin + 0.03 mM dopamine, (E) Cisplatin + 0.03 mM L-mimosine. No significant differences were observed in the proximal tubular histology.