FIGURE

Fig. 4

ID

ZDB-FIG-200817-24

Publication

Bottiglione et al., 2020 - Zebrafish IL-4-like Cytokines and IL-10 Suppress Inflammation but Only IL-10 Is Essential for Gill Homeostasis

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Fig. 4

Mutation of il10 induces morphological changes in the gills of adult zebrafish. (A) Representative images of sections (n = 3) of gills from wildtype, il4/13a^−/−, il4/13b^−/−, il4/13a;b^−/−, and il10^e46/e46 animals stained with H&E. Gill arch (A), filaments (F), and lamellae (L) are indicated. Lamellar epithelial cell alteration (asterisks), filament thickening (triangle), and arch thickening (arrow) are indicated. (B) Quantification of gill tissue damage in wildtype, il4/13a^−/−, il4/13b^−/−, il4/13a;b^−/−, and il10^e46/e46. Widespread alteration is observed in the gills of il10^e46/e46 animals. (C) Bar graph showing significantly enriched ontologies in gills of il10^e46/e46 animals. (D) Heatmap of NES for indicated gene sets generated by GSEA in gills of il10^e46/e46 compared with wildtype animals. (E) qPCR analysis showing transcript levels of il1β, il6, tnfα, and ifng1–2 in gills of il10^e46/e46 compared with wildtype animals. Gene expression was normalized to the expression of β−actin. Error bars represent SEM; n > 3, *p < 0.05.

Expression Data

Genes:	ifng1 il1b il6 tnfa
Fish:	WT il10^e46/e46
Anatomical Term:	gill
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	il4^umc18/umc18 il10^e46/e46 il13^umc17/umc17 il4^umc18/umc18; il13^umc17/umc17
Observed In:	gill gill filament gill lamella epithelial cell pharyngeal arch
Stage:	Adult

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Immunol.