Figure 3.

At 5 dpf, live zebrafish were adapted to a LED light, which was turned off at time 0 s (Hsieh et al., 2014). (A) Representative recordings from a control Purkinje cell (left) and a Purkinje cell expressing aR3H (right) before and after turning off the LED are shown. Time 0 s is indicated by dashed vertical lines. (B) Raster plots show firing of simple spikes (black) and complex spikes (red) before and after turning off the LED at time 0 s (dashed vertical lines) for control cells (left) and aR3H-expressing cells (right). Each row represents a different trial. Data were obtained from 8 control cells in 5 animals and 8 aR3H-expressing cells in 4 animals. (C) The average frequency of tonic firing per 100 ms interval was normalized to the average frequency calculated over the 10 s period before turning the LED off at time 0 s (dashed vertical lines). The fold-change in frequency from all trials was averaged and plotted versus time for control (left) and aR3H-expressing (right) Purkinje cells (blue lines; n = 8 control cells, n = 8 aR3H-expressing cells). The gray shaded areas represent the SEM. The solid red horizontal line indicates the original firing frequency before the imposition of sudden darkness.