Fig 2

(A) The levels of wild type psen2 allele mRNA in the psen2^S4Ter/+ fish (~700 copies) were significantly (p = 0.0039) lower than in their wild type siblings (~1,300 copies) under normoxia. Under hypoxia, the levels of wild type psen2 allele mRNA in both the psen2^S4Ter/+ fish (~1,000 copies) and their wild type siblings (~1,800 copies) were apparently up-regulated, but only the higher levels in the wild type fish showed a statistically significant increase (p = 0.0117) compared to the normoxic controls. (B) The levels of psen2^S4Ter allele mRNA in the psen2^S4Ter/+ fish (~700 copies) appeared to be lower than in the psen2^S4Ter/psen2^S4Ter fish (~1,000 copies) under normoxia. However, this comparison did not reach statistical significance (p = 0.0777). Under hypoxia, the levels of psen2^S4Ter allele mRNA in both the psen2^S4Ter/+ fish (~1,000 copies) and the psen2^S4Ter/psen2^S4Ter fish (~1,900 copies) were upregulated. This up-regulation in the psen2^S4Ter/psen2^S4Ter fish was clearly significant (p = 0.0002), while that in the psen2^S4Ter/+ fish was apparent, but not regarded as statistically significant (p = 0.1185). Data is shown as the mean ± SD. Values of p were determined by a two-way ANOVA followed by Tukey’s HSD test. The dqPCR raw data is given in S1 & S2 Tables in S1 File.