FIGURE

Fig. 3

ID

ZDB-FIG-200721-9

Publication

Carnovali et al., 2020 - Liquiritigenin reduces osteoclast activity in zebrafish model of glucocorticoid-induced osteoporosis

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Fig. 3

Analysis of TRAP activity in LTG-treated GIOP fish. (A) Percentage of TRAP-positive scales in adult zebrafish treated with different LTG concentrations with (PN vs 10pM LTG, p < 0.001; PN vs 100pM LTG, p < 0.001; PN vs 1 nM LTG) or without PN (CTR vs 10pM LTG, p < 0.001; CTR vs 100pM LTG, p < 0.001; CTR vs 1 nM LTG, p < 0.001; CTR vs 10 nM LTG, p < 0.001; CTR vs 100 nM LRG, p < 0,01). PN alone induced TRAP activity in 100% of scales (CTR vs 80 μM PN, p < 0.001) whereas 1 nM LTG suppressed PN-dependent TRAP activation (1 nM LTG + PN 80 μM vs PN 80 μM, p < 0.001, −90%). (B)Histochemical TRAP staining of scale treated with PN shows great resorption lacunae (purple staining) in all scales (100%) while only 10% of scales from 1 nM LTG+PN fish shows slight TRAP activity. (C) Biochemical TRAP analysis performed on scales confirms that 1 nM LTG treatment prevents the PN-dependent increase of TRAP activity (PN 80 μM vs CTR, p < 0.001, +99%; 1 nM LTG + PN 80 μM vs PN 80 μM, p < 0.001, −53%). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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