FIGURE

Figure 1

ID

ZDB-FIG-200709-3

Publication

Mackey et al., 2020 - Codon optimized Tol2 transposase results in increased transient expression of a crystallin-GFP transgene in zebrafish

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Figure 1

Codon-optimization improves transgenesis in surviving fish

A. Alignment of the C-terminal region of the Tol2 TPase protein and its homologs. Predicted NLS sequences are highlighted in yellow, predicted NES sequences are highlighted in blue. B. Image of zebrafish embryos 2 days post fertilization (dpf) after injection with pDestTol2pACryGFP and CO Tol2 TPase mRNA. Right embryo is positive for eye-localized eGFP expression. C. Results from embryo co-injection of pDestTol2pACryGFP with four different mRNA treatments. Survival rate is the proportion of fish that survived 2 dpf for a subset of the total injections (n = number of injections for which survival was measured). Overall efficiency was calculated by multiplying the survival rate by the percent of surviving fish that showed eye localized eGFP expression.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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