Figure 1

Codon-optimization improves transgenesis in surviving fish

A. Alignment of the C-terminal region of the Tol2 TPase protein and its homologs. Predicted NLS sequences are highlighted in yellow, predicted NES sequences are highlighted in blue. B. Image of zebrafish embryos 2 days post fertilization (dpf) after injection with pDestTol2pACryGFP and CO Tol2 TPase mRNA. Right embryo is positive for eye-localized eGFP expression. C. Results from embryo co-injection of pDestTol2pACryGFP with four different mRNA treatments. Survival rate is the proportion of fish that survived 2 dpf for a subset of the total injections (n = number of injections for which survival was measured). Overall efficiency was calculated by multiplying the survival rate by the percent of surviving fish that showed eye localized eGFP expression.
Acknowledgments
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