FIGURE

Fig. s4

ID
ZDB-FIG-200515-7
Publication
Shull et al., 2020 - The conserved and divergent roles of Prdm3 and Prdm16 in zebrafish and mouse craniofacial development
Other Figures
All Figure Page
Back to All Figure Page
Fig. s4

Loss of prdm3 does not lead to changes in pharyngeal arch cell proliferation or cell death. (A-D) Wildtype or prdm3−/− zebrafish embryos crossed into the Tg(sox10:EGFP) transgenic line were collected at 24 hpf and whole mount immunofluorescence was performed for phosphorylated histone H3 (A) or cleaved caspase 3 (B) to assess cell proliferation or apoptosis in the pharyngeal arches, respectively. Shown are lateral max projection images of the pharyngeal arches 1–7. (C-D) Quantification of the number of phosphorylated histone H3 positive cells (yellow) (C) or cleaved caspase 3 positive cells (yellow) (D) within the pharyngeal arches (n ​= ​6 embryos for each group). Abbreviations: eye (e), pharyngeal arches (1–7). Scale bar, 100 ​μm ​ns, not significant, Student’s t-test.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 461(2), Shull, L.C., Sen, R., Menzel, J., Goyama, S., Kurokawa, M., Artinger, K.B., The conserved and divergent roles of Prdm3 and Prdm16 in zebrafish and mouse craniofacial development, 132-144, Copyright (2020) with permission from Elsevier. Full text @ Dev. Biol.