FIGURE

Fig. 3

ID

ZDB-FIG-200514-43

Publication

Paatero et al., 2018 - Junction-based lamellipodia drive endothelial cell rearrangements in vivo via a VE-cadherin-F-actin based oscillatory cell-cell interaction

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Fig. 3

Oscillatory F-actin dynamics during remodeling of cell–cell junctions. a, b Still images from a movie (Supplementary Movie 4) showing JBL formation in the dorsal aorta of an EGFP-UCHD expressing 2dpf embryo (Tg(fli:Gal4ffubs3; UAS:EGFP-UCHDubs18)), shown in inversed contrast. b A magnification of the inset in a and the red dashed line indicates the site for kymograph in c. Arrows point to a JBL, seen as a local thickening of the junction. c Kymograph across the junction. Solid arrow denotes forward movement and dashed arrow backward movement of the junction. d Intensity plotting of a EGFP-UCHD JBL kymograph. e Scatter plot of the relative EGFP-UCHD intensity during forward and backward movements (n = 20 events, 4 movies). EGFP-UCHD intensity value in a forward movement was divided with intensity value during subsequent reverse movement. Non-parametric one sample Wilcoxon signed rank test was used as statistical test. Scale bars 5 µm

Expression Data

Gene:	EGFP
Fish:	ubs18Tg; ubs3Tg
Anatomical Terms:	blood vessel endothelial cell cell-cell junction blood vessel endothelial cell lamellipodium
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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