FIGURE

Fig. 5.

ID

ZDB-FIG-200509-39

Publication

Veerman et al., 2019 - Genetic variation in GNB5 causes bradycardia by augmenting the cholinergic response via increased acetylcholine-activated potassium current (I_K,ACh)

Other Figures

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Fig. 5.

Effect of I_K,ACh blockade on heart rate (HR) in gnb5-knockout zebrafish. (A) Experimental setup for high-speed imaging of the zebrafish heart. HR was recorded at 150 fps for 10 s. (B) Time schedule of drug treatment and CCh challenge in gnb5-knockout larvae. (C) Scatter dot plots with mean of HR of gnb5-knockout larvae 5 days post-fertilization at basal level, after treatment with 50 µmol l⁻¹ XEN-R0703 or DMSO, and upon application of 500 µmol l⁻¹ CCh. DMSO, n=13; XEN-R0703, n=22. **P<0.01, ***P<0.001, ****P<0.0001 (two-way repeated measures ANOVA followed by Bonferroni corrected pairwise comparisons). (D) Percentage of gnb5-knockout larvae that responded to 500 µmol l⁻¹ CCh with a decrease in HR of 0-25, 26-50, 51-75 or 76-100%. DMSO, n=13; XEN-R0703, n=22. Only 27% of gnb5-knockout larvae pretreated with XEN-R0703 displayed a pathological decrease in HR (51-75%, 76-100%), while over 80% of the not-pretreated gnb5-knockout larvae display the same decreased level of HR.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	gnb5a^{hu11433/hu11433}; gnb5b^{hu11443/hu11443}
Conditions:	chemical treatment: carbachol chemical treatment: carbachol
Observed In:	heart contraction
Stage:	Day 5

Phenotype Detail

Acknowledgments

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