FIGURE

Figure 1.

ID

ZDB-FIG-200421-63

Publication

Scaletti et al., 2020 - MutT homologue 1 (MTH1) removes N6-methyl-dATP from the dNTP pool

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Figure 1.

MTH1 catalyzes the hydrolysis of N6-methyl-dATP.A, MTH1 catalyzes the hydrolysis of N6-methyl-dATP to N6-methyl-dAMP and PP_i. B, time course hydrolysis of N6-methyl-dATP (1 mm) catalyzed by MTH1 (20 nm) was monitored by separation of reaction samples incubated 0–40 min at 22 °C on a Hypercarb column using HPLC coupled to MS. Reaction substrate and product was detected at 254 nm and the mass of the product N6-methyl-dAMP was clearly observed by mass detection. C, graph showing the fraction of N6-methyl-dATP and N6-methyl-dAMP in percent after various times of hydrolysis based on the respective area under the curve of the peaks in the corresponding HPLC chromatogram.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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