FIGURE

Fig. 1

ID
ZDB-FIG-200307-1
Publication
Horstick et al., 2020 - Molecular and cellular determinants of motor asymmetry in zebrafish
Other Figures
All Figure Page
Back to All Figure Page
Fig. 1

Individual larvae show persistent motor asymmetry during dark-induced circling.

a Swim trajectories during baseline illumination and after loss of light. Color scale: time (seconds). Arrowheads, orientation. Scale bar 20 mm. b NTA over 30-s intervals for larvae before/after loss of illumination. Individuals were classified as right- (cyan, N = 25) or left-biased (gray, N = 34) based on the first 30-s interval. Asterisk p < 0.05, d = 3.1, 0.71, 0.66, and 0.61 (first four time points in the dark), t test between groups. c NTA for 30-s light-off trials (black bars). Open circles at time 0 show individual larvae, and were used to classify as right- (cyan, N = 34) or left-biased (black, N = 34). Subsequent points show mean and standard deviation for left/right groups. Asterisk p < 0.05, t test between groups. d As for (c) during constant illumination (right, N = 29; left, N = 35). e Match Index during baseline illumination (yellow, N = 64) and after loss of illumination (gray, N = 68). Asterisk p < 0.05, r = 0.29, Mann–Whitney U test, and #p < 0.05, r = 0.76, one-sample permutation test against 0.5. f Visually isolated chamber. Right: time-lapse montage over 10 s following loss of illumination. Color: time (seconds). g, h Percentage of turns executed rightward (mean of four 10-s trials) after loss of illumination (g, gray, N = 89) or during constant illumination (h, yellow, N = 39). Red line: expected distribution, Monte Carlo simulation of unbiased larvae. i Rightward-turn preference over 24 h. Larvae with <33% of rightward turns at 6 dpf were classified as left-biased (black, N = 12), and those >66% as right-biased (cyan, N = 14). At 7 dpf, % rightward-turn use in L/R-classified groups. Repeated measures ANOVA for 7-dpf trials, the effect of 6-dpf first-trial direction F1,24 = 15.4, p < 0.001, η2p = 0.39. Asterisk p < 0.05 between groups. j Percentage trials with net rightward bias for larvae tested at 7 or 10 dpf (N = 30, 52), after left/right classification at 6 dpf. Asterisk p < 0.05, r = 0.61 and 0.60 (7 and 10 dpf), Mann–Whitney U test. Error bars: standard error of the mean. Box plots show median and quartiles with whiskers indicating 10–90%. Source data are provided as a Source Data file.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.