ZFIN ID: ZDB-FIG-200225-1
Belzunce et al., 2020 - The interplay of atoh1 genes in the lower rhombic lip during hindbrain morphogenesis. PLoS One   15:e0228225 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 10-13 somites to High-pec

Fig 1 Spatiotemporal analysis of <italic>atoh1a</italic> and <italic>atoh1b</italic> within the hindbrain.

A-E) Whole mount double in situ hybridization with atoh1a (green) and atoh1b (magenta) in wild type embryos from 14hpf to 42hpf. Dorsal views with anterior to the left. A’-E’) Reconstructed transverse views of dorsal views in (A-E) at the level indicated by the white arrow depicted in (A-E). Note that the expression of atoh1b is more lateral than atoh1a-cells. Dotted line corresponded to the neural tube contour. F-H) Whole mount double in situ hybridization with atoh1a (green) and atoh1b (magenta) on Tg[HuC:GFP] embryos from 24hpf to 42hpf, where HuC expression was displayed in white. Dotted line corresponded to the neural tube and the HuC-expression contours (only half of it). I-J) Embryos at 30hpf were double in situ hybridized with atoh1a (green) and atoh1b (magenta) and cell proliferation was assessed by anti-PH3 staining (white). Dorsal views with anterior to the left. I’-J’) Reconstructed transverse views of (I-J) at the level pointed by the white arrow in (I-J). Note atoh1a-cells underwent mitosis, whereas fewer atoh1b-cells did. Dotted line corresponded to the neural tube contour. K-K”) Tg[atoh1a:GFP] embryo after anti-PH3 (magenta) and DAPI (blue) staining. K’-K”) Reconstructed transverse views of the region framed in (K), which is a dorsal view with anterior to the left. This is an example of an apical atoh1a:GFP cell undergoing division (black asterisk) and lateral atoh1a:GFP cell that did not (white asterisk), with (K’) or without (K”) the red-PH3 staining. Note that atoh1a:GFP cell nuclei expressing PH3 are located in the apical region (black asterisks), whereas atoh1a:GFP cell nuclei negative for PH3 (most probably atoh1b-positive, white asterisk) are in the most lateral domain. L-L”) Tg[atoh1a:GFP] embryo incubated for 30min with BrdU (blue) and assayed for atoh1b in situ hybridization (magenta). Reconstructed transverse views with (L-L’) or without (L”) atoh1b-staining. White asterisks indicate atoh1b cells that did not incorporate BrdU. Dotted line corresponded to the neural tube contour. op, otic placode; ov, otic vesicle; r, rhombomere. Scale bars correspond to 50 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
atoh1a WT standard conditions 10-13 somites hindbrain dorsal-most region ISH
14-19 somites hindbrain dorsal-most region ISH
Prim-5 hindbrain dorsal-most region ISH
Prim-15 hindbrain medial region ISH
High-pec hindbrain dorsal region ISH
High-pec hindbrain medial region ISH
knu3Tg standard conditions Prim-5 hindbrain ISH
Prim-15 hindbrain ISH
High-pec hindbrain ISH
atoh1b WT standard conditions 10-13 somites otic placode ISH
14-19 somites hindbrain dorso-lateral region ISH
14-19 somites otic vesicle ISH
Prim-5 hindbrain dorso-lateral region ISH
Prim-5 otic vesicle ISH
Prim-15 hindbrain lateral region ISH
Prim-15 otic vesicle ISH
High-pec hindbrain lateral region ISH
hzm2Tg; pfu103Tg standard conditions Prim-15 hindbrain ISH
knu3Tg standard conditions Prim-5 hindbrain ISH
Prim-15 hindbrain ISH
High-pec hindbrain ISH
GFP hzm2Tg; pfu103Tg standard conditions Prim-15 hindbrain IHC
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One