FIGURE

Fig. 2

ID
ZDB-FIG-200130-2
Publication
Bai et al., 2020 - CRISPR/Cas9-mediated precise genome modification by a long ssDNA template in zebrafish
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Fig. 2

A genetic assay for comparing the efficiency of homology-directed repair using tyr mutant. a Table of template design schematics (left), attributes of the template (middle) and proportion of observed pigmented embryos in the tyr25de/l25del model (right). Embryos are analysed at 2 dpf after co-injection of zCas9 mRNA, tyr25de/l25del gRNA together with repair template. Number of embryos evaluated (n) exceeded 100 for each condition. b Phenotypic evaluation of embryos at 2 dpf into three groups according to number of pigmented cells: low rescue (1–20 pigmented cells), medium rescue (21–40 pigmented cells) and high rescue (more than 40 pigmented cells). Scale bar = 1 mm. c Statistics of HDR efficiency induced by different repair templates. zLOST: long single stranded template 299 bp, ssODN: single strand DNA oligonucleotides 105 bp, cdsDNA: circular double stranded DNAs 1527 bp (with two gRNA sites at both ends of the homologous arms), Ctl: without repair template. Number of embryos assessed (n) is shown for each group. X2-test (***p < 0.001). d Sequence analysis confirming that the larvae contained a correctly repaired tyr locus by zLOST. Correct insertion by HDR (green), PAM region (blue), target sites (underlined), Indels (red) are indicated.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
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