FIGURE

Fig. 3

ID

ZDB-FIG-200115-20

Publication

Leon et al., 2020 - Structural basis for adhesion G protein-coupled receptor Gpr126 function

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Fig. 3

Alternative splice isoforms of Gpr126 modulate ECR conformation.

a Schematic diagram of Gpr126 splice isoforms generated by including (+ss) or excluding (−ss) exon 6. Residues encoded by exon 6 are colored magenta. Gray asterisks indicate potential O-linked glycosylation sites and the black asterisk indicates a predicted N-linked glycosylation site. The conserved disulfide bond in the linker is colored yellow. b, c Negative-stain EM 2D class averages for −ss (b) and +ss (c) ECR constructs. Class averages are categorized according to similar orientations: (i, ii, iii, iv, v, and vi). (i, ii, iii, iv) are observed in both –ss and +ss isoforms. (vi) represents open-like conformations (>50° angle) that are observed only in the +ss isoform. (v) represents unidentifiable miscellaneous views. Scale bars (white) represent 50 Å. d Quantification of percentage of particles per category for both isoforms. e Representative individual particles for both isoforms. Yellow arrows point to particles which are not in a closed conformation. f ECR conformations based on negative-stain EM are depicted as cartoons. The splice site is shown in magenta. Black arrows with dashed lines indicate dynamic ECR conformation.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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