FIGURE

Fig. 4.

ID

ZDB-FIG-191230-398

Publication

Liao et al., 2019 - Targeted knockout of GABA receptor gamma 2 subunit provokes transient light-induced reflex seizures in zebrafish larvae

Other Figures

All Figure Page

Back to All Figure Page

Fig. 4.

Valproic acid (VPA) treatment alleviates the movement phenotype and neuronal hyperexcitability of gabrg2^−/−larvae. (A) Quantification of the maximum acceleration upon light stimulus before and after an acute VPA treatment of 200 µM for 2 h, showing the alleviating effect of VPA. (****P<0.0001, unpaired t-test.) (B) Representative swimming tracks (15 s following photic stimulus) showing that VPA exposure prevent the whirlpool behavior of gabrg2^−/− (5 dpf). (C,D) Calcium imaging and fluorescence quantification from gabrg2-KO Tg(neurod:GCaMP6f) larvae (5 dpf), showing that the neuronal hyperexcitability is relieved after VPA treatment (200 µM for 2 h). ot, optic tectum; cb, cerebellum. (****P<0.0001, ***P<0.001, **P<0.01, *P<0.05, two-way ANOVA).

Expression Data

Gene:	GCaMP
Fish:	gabrg2^{udm302/udm302}; icm05Tg
Conditions:	lighting conditions chemical treatment by environment: valproic acid, lighting conditions
Anatomical Term:	brain
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	gabrg2^{udm302/udm302} gabrg2^{udm302/udm302}; icm05Tg
Conditions:	chemical treatment by environment: valproic acid lighting conditions chemical treatment by environment: valproic acid, lighting conditions
Observed In:	brain brain transmission of nerve impulse motor behavior phototaxis
Stage:	Day 5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dis. Model. Mech.