Figure 2—figure supplement 1.

ptc2:Kaede and her12:Kaede embryos were photoconverted at 24 hpf (A), 48 hpf (B) and 72 hpf (C) and imaged 6 hr post-conversion. Each line (grey) represents Kaede^green fluorescent intensity along the mediolateral or dorsoventral axis (see Figure 2A) of one embryo. The red line denotes the average profile of all embryos (n = 4). The max intensity axes are 0–50%, while the DV/ML axes display the full extent of the transverse section. The position of the spinal canal in the dorsoventral profiles is denoted by a dotted line. (A) The signalling profiles during the ‘signalling activation’ phase. (B) The signalling profiles during the ‘signalling consolidation’ phase. (C) The signalling profiles during the ‘signalling termination’ phase. (D) The average profiles of all three phases plotted together. The relevant spinal canal position in the dorsoventral profile is marked by the matching coloured dotted line.