Fig. 4

9-HSA Induces RPCs Proliferation via the Activation of Canonical Wnt Signaling

Expression analysis of the c-myc and cyclinD1 Wnt targets at 2 and 3 days postfertilization (dpf) in DMSO-treated (respectively, (A), (E), (C), and (G)) and 9-HSA-treated retinae (respectively (B), (F), (D), and (H)).

(A–D) Comparative in situ hybridization revealed an increased expression of c-myc in the CMZ and in the central part of the 9-HSA-treated retinae in comparison to DMSO-treated control retinae at 2 and 3 dpf.

(E–H) Similarly to c-myc, comparative analysis of cyclinD1 expression in DMSO-treated and 9-HSA-treated retinae showed an increased expression of cyclinD1 in the retinae embryos injected with 9-HSA in comparison to DMSO control retinae.

(I) Quantitative real-time PCR of c-myc, cyclinD1, her4.2 and p27expression levels in 2 dpf 9-HSA-injected embryo heads versus DMSO-injected embryo heads (n = 3 for all conditions; her4.2 fold increase of 4.117 SEM ± 0.6289, p value = 0.0384; c-myc fold increase of 2.059 SEM ± 0.1231, p value = 0.0132; cyclinD1 fold increase of 1.814 SEM ± 0.1441, p value = 0.0299; p27 fold increase of 0.5461 SEM ± 0.1119, p value = 0.0557). Statistically significant was using a Student’s t test and depicted as: ^∗p value < 0.05; ns = nonsignificant. Scale bar, 50 μm.