ZFIN ID: ZDB-FIG-190822-26
Fischer et al., 2019 - FoxH1 represses miR-430 during early embryonic development of zebrafish via non-canonical regulation. BMC Biology   17:61 Full text @ BMC Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Term:
Stage Range: Sphere to 50%-epiboly
PHENOTYPE:
Fish:
Observed In:
Stage Range: Sphere to 50%-epiboly

Fig. 5

FoxH1 blocks maternal clearance of cd82b and jade1. ab RT-qPCR analysis of cd82b (a) or jade1 (b) in embryos at 50% epiboly with indicated genetic background. Massive reduction of expression is shown in MZsur mutants which cannot be rescued by injection of FHD-VP16 mRNA but is rescued via injection of foxH1WT mRNA. Error bars indicate standard error (SEM) from 2 biological replicates. Calculation of relative normalized expression, standard error, and significance was made with the Bio-Rad CFX Manager 3.1 software (***p < 0.001; n.s. p ≥ 0.05). For individual values, see also Additional file : Individual qPCR values. cf WISH for cd82b and jade1 in wild type embryos (ce) shows staining at sphere stage, but not at shield stage when miR-430 becomes active (c′, e′). In MZsur mutants (df), weak or no staining is visible for all stages indicating the negative role of FoxH1 in regulating miR-430 activity at early embryonic stages. Size bars 200 μm
 

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
cd82b WT standard conditions Sphere whole organism ISH
50%-epiboly whole organism RTPCR
Shield Not Detected whole organism ISH
foxh1m768/m768 standard conditions Sphere whole organism ISH
50%-epiboly whole organism RTPCR
Shield Not Detected whole organism ISH
jade1 WT standard conditions Sphere whole organism ISH
50%-epiboly whole organism RTPCR
Shield Not Detected whole organism ISH
foxh1m768/m768 standard conditions Sphere whole organism ISH
Shield Not Detected whole organism ISH
50%-epiboly whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
foxh1m768/m768 standard conditions Sphere whole organism cd82b expression decreased amount, abnormal
Sphere whole organism jade1 expression decreased amount, abnormal
50%-epiboly whole organism jade1 expression decreased amount, abnormal
50%-epiboly whole organism cd82b expression decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal BMC Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ BMC Biol.