FIGURE

Fig. 5

ID
ZDB-FIG-190822-26
Publication
Fischer et al., 2019 - FoxH1 represses miR-430 during early embryonic development of zebrafish via non-canonical regulation
Other Figures
All Figure Page
Back to All Figure Page
Fig. 5

FoxH1 blocks maternal clearance of cd82b and jade1. ab RT-qPCR analysis of cd82b (a) or jade1 (b) in embryos at 50% epiboly with indicated genetic background. Massive reduction of expression is shown in MZsur mutants which cannot be rescued by injection of FHD-VP16 mRNA but is rescued via injection of foxH1WT mRNA. Error bars indicate standard error (SEM) from 2 biological replicates. Calculation of relative normalized expression, standard error, and significance was made with the Bio-Rad CFX Manager 3.1 software (***p < 0.001; n.s. p ≥ 0.05). For individual values, see also Additional file : Individual qPCR values. cf WISH for cd82b and jade1 in wild type embryos (ce) shows staining at sphere stage, but not at shield stage when miR-430 becomes active (c′, e′). In MZsur mutants (df), weak or no staining is visible for all stages indicating the negative role of FoxH1 in regulating miR-430 activity at early embryonic stages. Size bars 200 μm
 

Expression Data
Genes:
Fish:
Anatomical Term:
Stage Range: Sphere to Shield

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Observed In:
Stage Range: Sphere to 50%-epiboly

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Biol.