Fig. 3

Regulation of Periodic Actin Polymerization Waves by the Cleavage Cycle Oscillator

(A) Images of the blastodisc of oocytes expressing DCLK-GFP and Utr-mCherry to mark microtubules and F-actin, respectively, during the first cell cycle.

(B) Schematic illustrating the embryonic cell-cycle oscillator and its regulation of bulk actin polymerization.

(C) Normalized bulk actin intensity in control (blue, N = 1 experiment, n = 2 oocytes, measured on 5 different stacks: z = 5), Wee1 inhibitor treated (red, N = 1, n = 2, z = 6), Chk1 overexpressing (Chk1-OE, green, N = 1, n = 2, z = 10) and Cdk1 inhibitor treated (black, N = 2, n = 6, z = 6) oocytes during the first 100 mpf. Arrowheads indicate peaks of bulk actin intensities.

(D) Periodicity of bulk actin oscillations for the conditions described in (C). ^∗p = 0.0253, ^∗∗p = 0.0095, ^∗∗∗∗p < 0.001, unpaired t test.

(E) Periodicity of bulk actin oscillations (same data as in C) and YGs movements for the conditions described in (C). For YG movements: control (N = 2, n = 6), Wee1 inhibitor (N = 2, n = 4), Chk1-OE (N = 2, n = 8), Cdk1 inhibitor (N = 2, n = 4).

(F) Images of mini-oocytes marking F-actin, obtained from the animal (A) half (top row) or vegetal (V) half (bottom row) of an oocyte. Panels boxed in gold correspond to the onset of the bulk actin polymerization wave.

(G) Bulk actin intensity in the A (red) and V (blue) mini-oocytes during the first 100 mpf. N = 1, n = 3 for each case.

(H) Onset of bulk actin polymerization waves in A and V mini-oocytes, their onset delay, and expected delay calculated from the speed of the polymerization wave as shown in Figure S3D. Box and whiskers, min to max. N = 4, n = 13 (for each A and V mini-oocytes).

^∗∗∗∗p < 0.001, Mann-Whitney test. Error bars, SEM, except in (E), SD.