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Fig. 4

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ZDB-FIG-190807-31

Publication

Neitzel et al., 2019 - Developmental regulation of Wnt signaling by Nagk and the UDP-GlcNAc salvage pathway

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Fig. 4

Inhibition of Nagk and N-glycosylation in zebrafishinduces cyclocephaly. (A) (Left) Injection of Nagk^T128M mRNA or 3-OMe-GlcNAc results in cyclopic zebrafish. (Right) Representative images of cyclopic zebrafish (5 days post fertilization) injected with Nagk^T128M mRNA. Animals were defined as cyclopic as previously described (Marlow et al., 1998). *ρ < 0.0253. (B) Injection of Nagk^T128M mRNA induces cyclopia, which is rescued by co-injection with GlcNAc, GlcNAc-6-P, GlcNAc-1-P, or UDP-GlcNAc. *ρ < 0.0127. (C) Injection of Ngly1mRNA also induces cyclopia. *ρ < 0.05. Results are aggregates of n ≥ 3 replicates. Injections were performed with 0.5 ng mRNA or 25 pmol sugar. Absolute numbers are indicated above bars. Significance was calculated using Fisher's exact test with Bonferroni correction.

Expression Data

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Antibody Labeling

Phenotype Data

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Acknowledgments

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Reprinted from Mechanisms of Development, 156, Neitzel, L.R., Spencer, Z.T., Nayak, A., Cselenyi, C.S., Benchabane, H., Youngblood, C.Q., Zouaoui, A., Ng, V., Stephens, L., Hann, T., Patton, J.G., Robbins, D., Ahmed, Y., Lee, E., Developmental regulation of Wnt signaling by Nagk and the UDP-GlcNAc salvage pathway, 20-31, Copyright (2019) with permission from Elsevier. Full text @ Mech. Dev.