Fig. S2

Habituation of dark flash response components. Related to Figures 2 and 3.

A) Habituation is evident in 8 different components of the response, each dot represents the mean response to

each stimulus (n=3120 larvae).

B) Histograms of the distribution of latencies observed in response to dark flashes. n = 392161 responses in

3120 larvae (left). Histograms of the bimodal distribution of latencies observed in response to taps. n = 44515

responses in 3120 larvae (right).

C) A “Compound Response” is defined as a movement where a large angle bend (Bend 1) is followed by a

period of partial relaxation of the bend, before performing a second large angle bend (Bend 2). Finally, the

larva fully relaxes and swims forward. In contrast, a “Simple Response” is characterized by a single large angle

bend (Bend 1), which fully relaxes and the larva swims forward.

C) Correlation matrix quantifying how correlated individual components of the dark flash response are in naïve

larvae (Spearman’s Rho). The matrix is sorted as to match Figure 3D. Note that the “Probability” component

can not be compared with the other components, since the remaining components can only be analyzed in

conditions when a larva responds to the flash